Table 1. Mean sample proportion of bacterial phyla by study group (Pp, primiparous and Mp, multiparous) and study day (S1, 3 weeks prior to calving; S2, 1–3 days after calving) in Roche 454 and Ion Torrent samples.
| Phylum | Platform | Study group (SG) | Study day (SD) | P-value | ||||
|---|---|---|---|---|---|---|---|---|
| Pp | Mp | S1 | S2 | PSG | PSD | PSG∗SD | ||
| Bacteroidetes | Roche 454 | 0.8450 | 0.7700 | 0.7640 | 0.8400 | ** | *** | *** |
| Ion Torrent | 0.7613 | 0.7032 | 0.6940 | 0.7610 | * | *** | *** | |
| Cyanobacteria | Roche 454 | 0.0040 | 0.0030 | 0.0060 | 0.0020 | *** | ** | |
| Ion Torrent | 0.0069 | 0.0050 | 0.0077 | 0.0047 | ||||
| Fibrobacteres | Roche 454 | 0.0110 | 0.0150 | 0.0140 | 0.0110 | ** | *** | *** |
| Ion Torrent | 0.0082 | 0.0115 | 0.0086 | 0.0108 | *** | |||
| Firmicutes | Roche 454 | 0.0800 | 0.1060 | 0.1200 | 0.0730 | *** | *** | |
| Ion Torrent | 0.1164 | 0.1420 | 0.1492 | 0.1142 | *** | |||
| Proteobacteria | Roche 454 | 0.0080 | 0.0200 | 0.0150 | 0.0140 | *** | ** | ** |
| Ion Torrent | 0.0096 | 0.0097 | 0.0105 | 0.0090 | ||||
| Spirochaetes | Roche 454 | 0.0010 | 0.0040 | 0.0030 | 0.0020 | ** | ||
| Ion Torrent | 0.0035 | 0.0073 | 0.0067 | 0.0044 | ||||
| Tenericutes | Roche 454 | 0.0050 | 0.0110 | 0.0120 | 0.0050 | * | ||
| Ion Torrent | 0.0094 | 0.0109 | 0.0136 | 0.0076 | ** | |||
| TM7 | Roche 454 | 0.0030 | 0.0050 | 0.0050 | 0.0040 | ** | * | * |
| Ion Torrent | 0.0050 | 0.0089 | 0.0093 | 0.0052 | * | ** | ||
Notes.
The magnitude of the p-values for the effect of study group (PSG), study day (PSD), and the interaction term (PSG∗SD) are shown on the right.
P < 0.001.
P < 0.01.
P < 0.05.