. 2016 Feb 4;4:e1599. doi: 10.7717/peerj.1599

Table 2. Mean sample proportion of bacterial genera by study group (Pp, primiparous and Mp, pluriparous) and study day (S1, 3 weeks prior to calving; S2, 1–3 days after calving) in 454 and Ion torrent samples.

Taxon	Platform	Study group (SG)		Study day (SD)		P-value
		Pp	Mp	S1	S2	P_SG	P_SD	P_SG∗SD
*Bacteroidetes*
Paraprevotellaceae	Roche 454	0.0040	0.0080	0.0040	0.0070
Paraprevotellaceae	Ion Torrent	0.0070	0.0070	0.0070	0.0070
Bacteroidales	Roche 454	0.0420	0.0720	0.0620	0.0540	^**	^***	^***
Bacteroidales	Ion Torrent	0.0430	0.0630	0.0560	0.0500
BF311	Roche 454	ND	ND	ND	ND
BF311	Ion Torrent	0.0020	0.0040	0.0040	0.0020
CF231	Roche 454	0.0130	0.0180	0.0150	0.0150
CF231	Ion Torrent	0.0160	0.0170	0.0150	0.0170
Prevotella	Roche 454	0.7200	0.5680	0.5820	0.6900	^**	^***	^***
Prevotella	Ion Torrent	0.6280	0.5270	0.5250	0.6170		^***	^***
Prevotellaceae	Roche 454	0.0040	0.0060	0.0030	0.0070	^*		^*
Prevotellaceae	Ion Torrent	0.0040	0.0050	0.0040	0.0040
RF16	Roche 454	0.0350	0.0430	0.0610	0.0230		^***	^***
RF16	Ion Torrent	0.0360	0.0420	0.0520	0.0290		^***
S24-7	Roche 454	0.0150	0.0360	0.0140	0.0340			^*
S24-7	Ion Torrent	0.0120	0.0190	0.0090	0.0200
YRC22	Roche 454	0.0100	0.0100	0.0130	0.0080
YRC22	Ion Torrent	0.0110	0.0110	0.0110	0.0110
*Firmicutes*
Mogibacteriaceae	Roche 454	ND	ND	ND	ND
Mogibacteriaceae	Ion Torrent	0.0020	0.0030	0.0030	0.0020
Butyrivibrio	Roche 454	0.0040	0.0020	0.0030	0.0030
Butyrivibrio	Ion Torrent	0.0070	0.0050	0.0060	0.0050
Clostridiales	Roche 454	0.0240	0.0290	0.0370	0.0180		^***	^***
Clostridiales	Ion Torrent	0.0330	0.0380	0.0460	0.0270	^*	^***	^*
Coprococcus	Roche 454	0.0020	0.0020	0.0030	0.0020
Coprococcus	Ion Torrent	0.0060	0.0030	0.0040	0.0050
Lachnospiraceae	Roche 454	0.0130	0.0140	0.0150	0.0120
Lachnospiraceae	Ion Torrent	0.0210	0.0190	0.0230	0.0180
RFN20	Roche 454	0.0040	0.0150	0.0130	0.0060	^**	^**
RFN20	Ion Torrent	0.0040	0.0150	0.0110	0.0090	^*
Ruminococcaceae	Roche 454	0.0060	0.0120	0.0140	0.0060		^**
Ruminococcaceae	Ion Torrent	0.0080	0.0210	0.0170	0.0120	^***
Ruminococcus	Roche 454	0.0040	0.0080	0.0070	0.0050
Ruminococcus	Ion Torrent	0.0100	0.0110	0.0110	0.0100
Selenomonas	Roche 454	ND	ND	ND	ND
Selenomonas	Ion Torrent	0.0030	0.0020	0.0010	0.0040
Succiniclasticum	Roche 454	0.0110	0.0070	0.0130	0.0060		^***
Succiniclasticum	Ion Torrent	0.0100	0.0070	0.0110	0.0070
Veillonellaceae	Roche 454	0.0020	0.0020	0.0010	0.0030
Veillonellaceae	Ion Torrent	0.0030	0.0040	0.0030	0.0040

Notes.

ND, Not Detected; The magnitude of the p-values for the effect of study group (P_SG), study day (P_SD), and the interaction term (P_SG∗SD) are shown on the right.

^***

P < 0.001.

^**

P < 0.01.

P < 0.05.