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. 2016 Feb 4;4:e1635. doi: 10.7717/peerj.1635

Figure 2. miR-27b directly targets the 3′ UTR of RUNX1 mRNA.

Figure 2

(A) Bioinformatic prediction of interaction between miR-27b and the 3′ UTRs of swine RUNX1. For each schematic, the upper sequence is the binding site of miR-27b in 3′ UTRs of swine RUNX1, the middle sequence is the mature miR-27b, and the lower sequence is the mutated sequence of 3′ UTR. The seed sequence is underlined. (B) Schematic drawing of the putative binding sites or mutations of miR-27b binding-sites in 3′ UTR of RUNX1 mRNA. The locations of the potential binding sites or their mutations are presented by blank boxes. (C) The RUNX1 luciferase reporter construct was co-transfected with miR-27b mimics (or negative control) or miR-27b inhibitors (or negative control) into PK-15 cells (normalized to the firefly luciferase activity). Data are expressed as relative luciferase activities to control. (D) Western blot analysis of RUNX1 in cells transfected with miR-27b mimics or mimics control. Data represent means ± S.D. of three independent experiments. ∗P < 0.05 in comparison with the control. ∗∗P < 0.01 in comparison with the control.