Table 1. RT-PCR validation using 3 housekeeping and 6 differentially expressed genes in quiescent hydrated and hydrated-RD Globodera pallida eggs.
| Stages * | |||||
|---|---|---|---|---|---|
| Probes | Gene | Primers | BLAST hit | M | Q |
| CUST_3830, | GPLIN_000489300 | F: 5′CCCATAAGTGCCCAATTTGT3′ | No match | 2.90 | 2.1 |
| CUST_3831, | R: 5′TCATCTTTGGCTGCAGAATG3′ | ||||
| CUST_3832 | |||||
| CUST_40764, | GPLIN_000340000 | F: 5′CCAAACCCTTCGAAGATCAA3′ | dimethylaniline monooxygenase | 6.43 | 2.92 |
| CUST_40765, | R: 5′GCCACTTTGCTAAGCTCCAC3′ | ||||
| CUST_40766 | |||||
| CUST_34634, | GPLIN_001125100 | F: 5′GAGATGCTCCCATCGACTGT3′ | ph domain-containing protein 2 | 2.50 | 1.95 |
| CUST_34635, | R: 5′GCGTGTAATGGGAGCTGAAT3′ | ||||
| CUST_34636 | |||||
| CUST_684, | GPLIN_001330000 | F: 5′TTCAATTCCTTTTCCGGATG3′ | ras guanyl-releasing protein 3 | 2.43 | 1.7 |
| CUST_685, | R: 5′TGCCATCAGCGTGTTAAAAG3′ | ||||
| CUST_686 | |||||
| CUST_15290, | GPLIN_000723500 | F: 5′TGACATTGAGCCTGAACTGC3′ | haloacid dehalogenase subfamily variant 3 with third motif having dd or ed | 6.14 | 8.57 |
| CUST_15291, | R: 5′TGCCAGAAGAACGGAGAGAT3′ | ||||
| CUST_15292 | |||||
| probe003464, | GPLIN_000127800 | F: 5′AATGAGCATTCCGAGTGGAC3′ | No match | 2.76 | 2.1 |
| probe003466, | R: 5′TGGTCTTTTGTCCGGGTTAG3′ | ||||
| probe006774, | |||||
| probe006776, | |||||
| CUST_13407, | |||||
| CUST_13408, | |||||
| CUST_13409 | |||||
| Housekeeping genes** | |||||
| CUST_47827, | GPLIN_000606600 | F: 5′CACACAGATGGGGTGAGATG3′ | mitotic checkpoint protein bub3 | – | – |
| CUST_47828, | R: 5′GTACACTCTGTCCCCGCATT3′ | ||||
| CUST_47829 | |||||
| probe004575, | GPLIN_000738000 | F: 5′CCGCTGGACTTGTTGGTAAT3′ | anaphase-promoting complex subunit 1 | – | – |
| probe004576, | R: 5′TAAGCTGCAGCACATCCAAC3′ | ||||
| probe009599, | |||||
| CUST_15991, | |||||
| CUST_15992, | |||||
| CUST_15993 | |||||
| CUST_25109, | GPLIN_000931300 | F: 5′GCCAATGTGTTGATCACAGG3′ | No match | – | – |
| CUST_25110, | R: 5′TCGGTACCACAAAGTGACCA3′ | ||||
| CUST_25111 | |||||
Notes.
*
M, microarray; Q, QRT-PCR.
**
Genes with expression not significantly modified in the microarray analysis after ANOVA on genes which passed the filtering criteria, with p-value cutoff ≤0.05 and Bonferroni multiple testing correction and taking the average of all designed probes for the specified gene.