Figure 3.

Comparison between (i) spray based (Bruker ImagePrep system) and (ii) vacuum sublimated DHB matrix in MALDI imaging. (a1) 30 gL⁻¹ DHB (in 70% ACN solution) was used. Typical instrumental parameters include: 0.8 s spray, 10 s incubation, 70 s drying, and 60 spray cycles. (a2) 300 mg DHB was used. Major parameters include: ~120°C heating temperature, 0.05 Torr vacuum pressure, 10–11 min sublimation, matrix density 0.05 mgcm⁻². (b1,b2): DHB crystal sizes resulting from Bruker ImagePrep (b1) and sublimation (b2), scale bar: 10 μm. Figures were adapted with the permission from Ref. (Hankin et al., 2007; Franceschi et al., 2012).