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. 2016 Mar;22(3):339–349. doi: 10.1261/rna.054973.115

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© 2016 Foretek et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Both exonucleolytic 3′ end maturation pathway and chaperone function of Lhp1 influence the precursor pattern of tested tRNAs. (A–C) Wild-type and indicated mutant strains were grown at 30°C to exponential phase and then shifted for 2 h to 30°C or 37°C and/or YPGly. RNA was isolated and analyzed by Northern hybridization with probes specific to tRNA_UAU^Ile, tRNA_UUU^Lys, and tRNA^Tyr. Ethidium bromide–stained 5.8S rRNA was used as a loading control. The major and minor forms of pre-tRNA precursors that accumulate under stress conditions are indicated by solid and dashed arrows, respectively. (D) Calculation of ratios between various forms of pre-tRNA (specified in the legend to Fig. 1) from at least three biological replicates.