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. 2016 Mar;22(3):416–427. doi: 10.1261/rna.055178.115

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© 2016 Ruminski et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — Mss116p promoted equilibration between H1 and AltH1 helices in vivo. (A) HP210-310 has the potential to adopt a functional structure with an H1 helix that has a calculated free energy of −17.3 kcal/mol (72.4 kJ/mol) or a nonfunctional structure with a 3′AltH1 that has a calculated free energy of −20.5 kcal/mol (85.8 kJ/mol). Chimeric mRNAs with HP210-310 were coexpressed in yeast with (B) active Mss116p (Δmts), (C) catalytically inactive Mss116p (ΔmtsE268Q), or (D) empty vector. HP210-310 chimeric mRNAs in yeast with active Mss116p exhibited eightfold lower intracellular cleavage rates than HP210-310 chimeric mRNAs that were coexpressed with catalytically inactive Mss116p (ΔmtsE268Q) or empty vector. Plots display results from a representative pair of experiments with functional (▪) and mutationally inactivated (□) chimeric HP210-310 mRNAs. Reported values represent the mean and standard deviation obtained from two or more pairs of experiments.