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. 2016 Mar;22(3):416–427. doi: 10.1261/rna.055178.115

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© 2016 Ruminski et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 6. — Mss116p did not alter RNA folding nonspecifically. (A) HP210-510 has the potential to adopt a functional structure with an H1 helix that has a calculated free energy of −17.2 kcal/mol (72.0 kJ/mol) or a nonfunctional structure with a 5′AltH1 that has a calculated free energy of −20.2 kcal/mol (84.5 kJ/mol). Chimeric mRNAs with HP210-510 were coexpressed in yeast with (B) active Mss116p (Δmts), (C) catalytically inactive Mss116p (ΔmtsE268Q), or (D) empty vector. Mss116p expression had no detectable effect on HP210-510 self-cleavage activity. Plots display results from a representative pair of experiments with functional (▪) and mutationally inactivated (□) chimeric HP210-510 mRNAs. Reported values represent the mean and standard deviation obtained from two or more pairs of experiments.