Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar;22(3):428–442. doi: 10.1261/rna.053942.115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Harnisch et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

FIGURE 8. — The Trf4-1 protein is localized in the cytoplasm. (A) Confocal images of S2 cells transiently expressing the C-terminally YFP-tagged Trf4-1 (green). A wide cytoplasmic distribution of the protein is depicted in the upper panel. The two lower panels show the enrichment of the protein in small cytoplasmic foci. DAPI was included in the mounting medium to label the nuclei (blue). (B) Trf4-1-YFP foci are not P bodies. S2 cells transiently expressing Trf4-1-YFP (green) were stained with an antibody against Me31B. The secondary antibody was Cy3 labeled (red). Cells containing a wide distribution (upper panel) and cytoplasmic foci (middle panel) of Trf4-1-YFP are shown. An untransfected cell was included as a negative control (lower panel). DAPI staining is shown in blue. Note that plasmid yields of the original Trf4-1 clone and the Trf4-1-YFP clone were always poor, and cells containing the latter either failed to grow in liquid culture or did not grow to normal densities. As this suggests selection pressure on the plasmids, the correct sequence of the Trf4-1-YFP clone was verified in the preparation used for the transfection experiment shown here.