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. 2016 Mar;22(3):456–466. doi: 10.1261/rna.054452.115

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© 2016 Beznosková et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — Cytosine immediately following any stop codon interferes with the eRF1 decoding in vivo. (A) Stop codon readthrough measured at all four UGA-N termination tetranucleotides in wt cells (SUP45) and two eRF1 mutants (sup45-M48I and sup45-Y410S). The 74D-694, L2327, and L2521 strains were grown in SD and processed for stop codon readthrough measurements using standard dual luciferase readthrough reporter constructs YEp-R/T-CAAC-L; YEp-R/T-UGAC-L; PBB75; PBB76; and PBB77, as described in Materials and Methods. Readthrough values are represented as mean ± SD from quintuplicates (n = 5) and each experiment was repeated at least three times. (B) Normalization of readthrough measurements from panel A; values measured for UGA-U of each of the four strains were set to one. (C) Same as in panel A, except that all four UAA-N termination tetranucleotides were examined. (D) Same as in panel A, except that all four UAG-N termination tetranucleotides were examined.