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. 2014 Dec 24;25(1):4747. doi: 10.4081/ejtm.2015.4747

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig 3. — Comparison of micron- and nanometre-scale morphologies in adult mammalian cardiac t-systems. Transverse (i) Confocal and (ii) dSTORM images blurred with a 2D Gaussian PSF equivalent to a confocal PSF (left panels) and super-resolution image (right). Shown myocytes of (A) C57-BL/6 mouse and (B) Wistar rat were stained with a combination of NCX1 and CAV3. Fixed ventricular tissue sections from (C) New Zealand White rabbit, (D) Human (54-year old donor with normal echocardiogram) and (E) Horse (12-year old female New Forest pony) were stained with fluorescent wheat germ agglutinin to visualise the t-tubules. Note the nanometre- and micron-scale t-tubule dilatations in mouse and rabbit myocytes respectively (arrowheads). Cell surface in each example is indicated by asterisks. Scale bars: i-panels: 2 µm; ii: 1 µm.