. 2016 Feb 10;11(2):e0148438. doi: 10.1371/journal.pone.0148438

Table 1. Summary of PCR primer pairs.

Name	Annealing Temperature (°C)	Mean product size	Primer	Sequence (5’ to 3’)
β-actin	55	290	forward	`GTGACGTTGACATCCGTAAAGACC`
			reverse	`TCAGTAACAGTCCGCCTAGAAGCA`
FasL	55	231	forward	`CTGGGGATGTTTCAGCTCTTC`
			reverse	`CTTCACTCCAGAAAGCAGGAC`
FasR	60	384	forward	`ATGCTGGGCATCTGGACCCTCCTA`
			reverse	`TCTGCACTTGGTATTCTGGGTCCG`
IFN-β	55	174	forward	`AGTAGGCGACACTGTTCGTG`
			reverse	`AGCCTCCCATTCAATTGCCA`
IL-1β	55	219	forward	`AGCCATGGCAGAAGTACCTG`
			reverse	`TCCATGGCCACAACAACTGA`
TLR1^*	55	517	forward	`TCTGGTACACGCATGGTC`
			reverse	`ATGGGTGGGAAACTGAAT`
TLR2^*	55	264	forward	`AACTTACTGGGAAATCCTTAC`
			reverse	`AAAAATCTCCAGCAGTAAAAT`
TLR3^*	55	131	forward	`GCATTTGTTTTCTCACTCTTT`
			reverse	`TTAGCCACTGAAAAGAAAAAT`
TLR4^*	55	106	forward	`CGAGGAAGAGAAGACACCAGT`
			reverse	`CATCATCCTCACTGCTTCTGT`
TLR5^*	55	383	forward	`AGCTTCAACTATATCAGGACA`
			reverse	`TGGTTGGAGGAAAAATCTAT`
TLR6^*	55	123	forward	`CTTCCATTTTGTTTGCCTTAT`
			reverse	`AGCGGTAGGTCTTTTGGAAC`
TLR7^*	55	149	forward	`AAACTCCTTGGGGCTAGATG`
			reverse	`AGGGTGAGGTTCGTGGTGTT`
TLR8^*	55	246	forward	`CTGTGAGTTATGCGCCGAAGA`
			reverse	`TGGTGCTGTACATTGGGGTTG`
TLR9	55	242	forward	`GTTGCAAGGCTGTGGTGAAG`
			reverse	`CTGGATAGCACCAGTAGCGG`
TLR10^*	55	279	forward	`AGAAGAAAGGGAACTGATGAC`
			reverse	`CCTGCCAGTAAATACCAAGT`
TNF-α	55	109	forward	`AACATCCAACCTTCCCAAACG`
			reverse	`GACCCTAAGCCCCCAATTCTC`

* primer sequences were chosen from [37]