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. 2015 Dec 12;38(1):21–26. doi: 10.1002/bies.201500131

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© 2015 WILEY Periodicals, Inc.

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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The relationship between frameshift efficiency and mRNA abundance. The mathematical relationship between ‐1 PRF efficiency and mRNA abundance was determined using frameshift signals derived from the yeast EST1, EST2, STN1, and CDC13 mRNAs. These were cloned into a reporter gene and cellular mRNA steady‐state abundances were plotted relative to the abundance of the reporter without frameshift signals. Plotting of these data fit to the logarithmic function f(x) = e^−0.05x (adapted from 22).