Figure 6.

Stable and transient expression of CMG2‐Fc synonymous codon variants. (a) Transient expression of CMG2‐Fc gene variants in Nicotiana spp. by A. tumefaciens syringe infiltration (OD600 = 0.1). A. tumefaciens carrying the P19 post‐transcriptional gene silencing suppressor was co‐infiltrated into N. benthamiana leaves. Leaf samples were harvested 7 days postinfiltration, and CMG2‐Fc expression was measured by ELISA. (b and c) N. tabacum was transformed with either the codon‐optimized CMG2‐Fc gene (b) or the RNA‐optimized sequence (c). Approximately 120 individual T0 plants were screened for each transformation. Plants were screened by ELISA at 3‐week intervals until flowering stage. Each bar represents a plant (only expressing plants are shown). Grey bar, average expression. Orange bar, maximum expression detected in that plant.