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. Author manuscript; available in PMC: 2016 Feb 10.
Published in final edited form as: Biochemistry. 2015 Apr 16;54(16):2644–2658. doi: 10.1021/acs.biochem.5b00113

Figure 3.

Figure 3

Dead-end inhibition analysis of AANATL7. (A) Velocities measured at a fixed concentration of histamine (520 μM), varying the concentration of acetyl-CoA, and varying the concentration of the inhibitor, oleoyl-CoA: 0 nM (●), 200 nM (○), and 600 nM (▼) (Ki = 200 ± 10 nM). (B) Velocities were measured at a fixed concentration of acetyl-CoA (29 μM), varying the concentration of histamine, and varying the concentration of the inhibitor, oleoyl-CoA: 0 nM (●), 200 nM (○), and 600 nM (▼) (Ki = 410 ± 10 nM). (C) Velocities measured at a fixed concentration of histamine (520 μM), varying the concentration of acetyl-CoA, and varying the concentration of the inhibitor, tyrosol: 0 μM (●), 650 μM (○), and 1.3 mM (▼) (Ki = 620 ± 20 μM). (D) Velocities measured at a fixed concentration of acetyl-CoA (29 μM), varying the concentration of histamine, and varying the concentration of the inhibitor, tyrosol: 0 μM (●), 650 μM (○), and 1.3 mM (▼) (Ki = 430 ± 20 μM).