SPR responses at flow 100 μL/min for association (0–300 s) and dissociation (300–600 s) of AAHSA for protein films on an SPR chip. Different concentrations of AAHSA were injected: (a) 0 μg/mL, (b) 0.15 μg/mL, (c) 0.30 μg/mL, (d) 0.60 μg/mL, (e) 1.2 μg/mL, and (f) 1.8 μg/mL), and SPR signals were obtained. Chips were immobilized with (A) HSA and (B) GOx.