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. Author manuscript; available in PMC: 2016 Dec 10.
Published in final edited form as: J Control Release. 2015 Aug 6;219:237–247. doi: 10.1016/j.jconrel.2015.08.011

Figure 2.

Figure 2

Figure 2

Mechanisms of (A) luciferase/GFP based screening. Effective SMIR binds mature miRNA and further inhibit the binding of this miRNA to miRNA target sequence, leading to the expression of Luciferase/GFP. (B) Molecular beacon-based screening. Effective SMIR binds mature miRNA and further inhibit the activity of Dicer. The 5′-fluorophore (F) and 3′-quencher (Q) attach to each other and no signal can be detected. (C) structure-based design. Effective SMIR binds to the binding pocket of certain miRNA and further prevent this miRNA to bind miRNA target sequence. (D) Peptides or peptoids screening. Effective SMIR (peptides or peptoids) binds specific miRNA and further prevent this miRNA to bind miRNA target sequence. SMIR, small molecule inhibitor of miRNA.