Fig 5. Reduced VEGF lowers surfactant production.

(A, B) Representative images of immunofluorescence staining for cell specific markers T1α (Type I pneumocytes), SPC (Type II pneumocytes), and CC10 (Club cells) show similar distribution, frequency, and percentage of each cell type in both control mice (A) and mutant mice (B). (C) Western blot data for pro-SPC and pro-SPB. (D) Mutant mice produce significantly less pro-SPC, a downstream target of VEGF-R activation. (E) Decreased levels of pro-SPB seen in mutant mice were not significant. Scale bars represent 50 μm. Data are expressed as mean ± SD, *P<0.05.