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. Author manuscript; available in PMC: 2017 Feb 8.
Published in final edited form as: ACS Biomater Sci Eng. 2015 Dec 23;2(2):241–250. doi: 10.1021/acsbiomaterials.5b00458

Figure 4.

Figure 4

CS released into cell culture media over a seven day culture period. CS release was measured every two days (Figure 4A) and measured as a culmination of seven day culture period (Figure 4B). CS loss was measured by a DMMB assay. The addition of mAGC (HA binding), has a significant effect on preventing loss of CS from the scaffolds (p<0.01). * denotes statistical significance between explants treated with mAGC after aggrecan depletion and explants left untreated. Bars represent average ± SEM (n=9).