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. 2016 Feb 10;12(2):e1005440. doi: 10.1371/journal.ppat.1005440

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© 2016 Nawaz-ul-Rehman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 8 — (A) A yeast cell replicating TBSV repRNA was imaged based on Alexa Flour 647 (for p33 replication protein) and for ATTO488-phalloidin, which detect actin filaments. The bars represent 1 μm. The boxed area represents enlargement of the image to visualize the localization of actin and p33 replication protein in yeast. (B) Yeast cells free of viral components (top images) or replicating TBSV repRNA (bottom images) were imaged based on ATTO488-phalloidin, which detect actin filaments. The bars represent 1 μm. The images in both panels were prepared by a Nikon Super Resolution Microscope N-STORM and image processing was performed using NIS-element software.