Table 2. 3D liver model setups.

3D liver system building blocks	Setup 1 Single hepatocyte	Setup-2 Double hepatocyte	Setup-3 LSEC-double hepatocyte	Setup-4 LSEC-hepatocytea
COL matrix Ib	650 μl	650 μl	650 μl	650 μl
Hepatocytes I	4.105	4.105	4.105	4.105
Incubation	Overnight	Overnight	Overnight	Overnight
COL matrix IIb	150 μl	175 μl	175 μl	150 μl
Hepatocytes II	X	8.105	8.105	X
Incubation	X	Overnight	Overnight	X
COL matrix IIIb	X	150 μl	175 μl	X
LSEC	X	X	3.105	3.105
Incubation	2 days	Overnight	2 days	2 days
Time to build the system	3 day	3 days	4 days	3 days

footnotes:

^a- susceptible to HBV infection;

^b- solution (1mg/ml)