Fig 7. IE2 is degraded in the absence of its E3 ligase activity.

Wild type (WT) IE2 and IE2C230S (containing a RING domain mutation)-expressing recombinant viruses were transduced into Vero E6 cells together with KNK437 treatment at various concentrations. The cell extracts were collected at 48 hpt for the detection of IE2 protein levels.