Fig 7. Viral systemic infection is significantly delayed in the nonbranched ER network rhd3 mutant of A. thaliana compared with the Col-0 wild-type (WT).

(A and B) Symptoms of WT and rhd3-8 plants inoculated with mock (A) or TSWV (B) at 15 dpi. (C) Disease development was delayed in rhd3-8 mutant compared with WT after inoculation with TSWV. (D) Immunoblots of extracts from leaves of WT and rhd3-8 plants after systemic infection with TSWV and probing with monoclonal antibodies against TSWV nucleocapsid at 15 dpi. Protein loading is indicated by Ponceau S staining. The accumulation of TSWV in WT and rhd3-8 plants was quantified.