Table 1. Cell-to-cell movement of TSWV NSm in leaf epidermis of Nicotiana benthamiana after bombardment.
Bombarded plasmid | Protein size (kDa) | Total no. of signal clusters | No. of clusters with 1 cell (% of total) | No. of clusters with ≥2 cells (% of total) | P-value b |
---|---|---|---|---|---|
GFP-GFP | 54.0 | 53 | 53 (100%) a | 0 | |
NSm-GFP | 60.9 | 110 | 52 (47.3%) | 58 (52.7%) | < 0.001 |
NSm 133-135D -GFP | 60.9 | 84 | 84 (100%) | 0 | |
NSm 177-179D -GFP | 60.9 | 92 | 92 (100%) | 0 | |
NSm 4A/5A -GFP | 60.9 | 106 | 106 (100%) | 0 | |
NSm 230A/232A -GFP | 60.9 | 98 | 98 (100%) | 0 |
a Signal clusters comprise fluorescent cells, indicating presence of GFP fusion protein.
b P-values were calculated using unpaired two-tailed Student t-test.