Table 2. BFA disruption of the interconnected ER network inhibited cell-to-cell movement of TSWV NSm in Nicotiana benthamiana.

Bombarded plasmid	Treatment	Total foci	Number of total signal clusters (Percentage of total)
			1 cell/cluster	2 cells/cluster	3 cells/cluster	≥4 cells/cluster	P-value b
NSm-GFP	DMSO	58	36 (62.1%) a	6 (10.3%)	6 (10.3%)	10 (17.3%)
	BFA c	55	49 (89.1%)	4 (7.3%)	2 (3.6%)	0	<0.001
GFP	DMSO	136	118 (86.8%)	17 (12.5%)	1 (0.7%)	0
	BFA	202	180 (89.1%)	20 (9.9%)	2 (1.0%)	0	>0.05

^a Signal clusters comprise fluorescent cells showing the presence of GFP or GFP fusion protein.

^b P-values were calculated using the unpaired two tailed Student t-test.

^c BFA was used at 20 μg/mL.