Table 1. Thermodynamic parameters for mismatch next to labelled thymidine (one parameter for 5’ side mismatch NTE/AN (ATE/AV, CTE/AH, GTE/AD, TTE/AB), and one parameter for 3’ side mismatch TEN/NA (TEA/VA, TEC/HA, TEG/DA, TET/BA)).
nearest neighbor | ΔΔH° | ΔΔS° | ΔΔG°37 | ΔΔG°60 |
---|---|---|---|---|
(5' to 3' / 5' to 3') | [kcal mol-1] | [cal mol-1 K-1] | [kcal mol-1] | [kcal mol-1] |
NTE/AN | -3.6 ± 1.4 | -4.4 ± 2.4 | -2.3 ± 0.4 | -2.3 ± 0.3 |
TEN/NA | -2.9 ± 1.5 | -3.2 ± 2.6 | -2.0 ± 0.5 | -2.2 ± 0.3 |