Table 1. Puccinia striiformis f. sp. tritici candidate effectors analysed in this study.
| protein ID a | tribe b | protein length c | SP length c | cys d | gene expression be | similar proteins f |
|---|---|---|---|---|---|---|
| PST05258 | 54 | 256 | 21 | 0 | H | Pgt (3) |
| PST15391 | 54 | 256 | 19 | 0 | H5, I | Pgt (3) |
| PST11721 | 219 | 250 | 24 | 1 | H5, I | Pgt (1) |
| PST18220 | 238 | 110 | 19 | 0 | H5, I, TC | - |
| PST18221 | 238 | 112 | 20 | 0 | H1, I, TC | - |
| PST15642 | 308 | 102 | 21 | 3 | H5, I, TC | - |
| PST18447 | 318 | 146 | 24 | 4 | H5, I | Pgt (1), Mlp (1), Hv (1) |
| PST02549 | 320 | 297 | 21 | 1 | H5, I | - |
| PST05023 | 351 | 281 | 21 | 1 | H1, I10, TC | Pgt (1) |
| PST12160 | 354 | 168 | 19 | 1 | H5, I10 | - |
| PST10977 | 403 | 171 | 26 | 13 | H5, I | Pgt (1) |
| PST03196 | 478 | 206 | 25 | 2 | H5, I | Pgt (1) |
| PST05302 | 593 | 160 | 20 | 0 | H1, I, TC | - |
| PST15964 | 678 | 128 | 18 | 0 | H1, I | - |
| PST08468 | 1176 | 206 | 20 | 0 | H5, I | - |
| PST05006 | 1217 | 201 | 22 | 3 | H1, I | - |
a Protein IDs were adapted from [20] by removing the isolate ID for simplicity. Full-length protein IDs are indicated in Sheet A in S1 Table.
b Data mined from [20].
c Number of amino acids are indicated; SP: predicted signal peptide
d Number of cysteine residues in the mature form of the protein (i.e. without the signal peptide)
e H: transcripts were identified in isolated haustoria; H5: transcripts were in the top 5% of transcripts detected in isolated haustoria; H1: transcripts were in the top 1% transcripts detected in isolated haustoria; I: transcripts were identified in wheat tissues during infection; I10: transcripts were in the top 10% transcripts detected in wheat tissues during infection; TC: transcript accumulation was detected by RTq-PCR during a time-course infection of wheat.
f Pgt: Puccinia graminis f sp tritici; Mlp: Melampsora larici-populina; Hv: Hemileia vastatrix.