Fig 4. Immuno-histochemistry of intracellular albumin on 9^th day of hepatocyte cultures.

Hepatocyte monoculture (left), co-culture with NIH-3T3 J2 (middle) and monoculture with F-CM (55 mg/ml, right) on day 9 were fixed and permeabilized using paraformaldehyde and 0.1% Triton X-100, respectively. The fixed samples were then stained with anti-rat albumin primary antibody and Alexa Fluor 488 conjugated secondary antibody (green). Nucleus was counter-stained with DAPI (blue). Scale bars: 200 μm