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. Author manuscript; available in PMC: 2017 Feb 9.
Published in final edited form as: Cell Metab. 2015 Dec 24;23(2):344–349. doi: 10.1016/j.cmet.2015.12.008

Figure 2. A stable FGF21 analog decreases saccharin preference in mice and monkeys.

Figure 2

(A) Two bottle preference assay with 0.1% saccharin in diet-induced obese mice administered either PF-05231023 (10 mg/kg) or vehicle on days 0, 3, 7 and 10.

Data are shown as the mean ± S.E.M.; n = 8/group. **p < 0.01, ***p < 0.001 versus vehicle group.

(B) Two bottle preference assay with 0.2% saccharin in obese cynomolgus monkeys administered either PF-05231023 (n=8; 10 mg/kg) or vehicle (n=7) on days 1, 4 and 7. Data are presented as mean percentage change in saccharin water intake ± S.E.M. for vehicle-treated (closed blue circles) and PF-05231023-treated (open red circles) monkeys. Solid lines are locally weighted scatterplot smoothing fits to the means of percent change. Mixed effect modeling fitted to these longitudinal data using R, version 3.1.2 (Pinheiro et al., 2013), showed a significant difference (p = 0.003) between groups. Number of days after first treatment, treatment type, and the interaction term between treatment groups and time were specified as fixed effects and monkey labels as a random effect.