Figure 2. PTH treatment elevates the thermogenic gene program.
(A) Primary cultures of inguinal adipocytes were treated with 10 ng/ml PTHrP(1-34), 10 ng/ml PTH(1-34) or 25 ng/ml PTH(1-84) for 2 hr (n = 3).
(B-D) Mice received a single dose of PTH(1-34) (1 mg/kg body weight; SubQ) and were sacrificed 2 hr later (n = 6). Gene expression changes were determined by RT-qPCR. The values are mean ± SEM. *P < 0.05, **P < 0.005, ***P < 0.0005.