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. Author manuscript; available in PMC: 2017 Feb 9.
Published in final edited form as: Cell Metab. 2015 Dec 6;23(2):315–323. doi: 10.1016/j.cmet.2015.11.003

Figure 4. PTH and PTHrP are unable to induce thermogenic genes in the absence of PTHR.

Figure 4

(A) Primary adipocytes from wild type and mutant cells were treated with 10 ng/ml PTHrP(1-34), 10 ng/ml PTH(1-34) or 10 nM Norepinephrine (NE) for 2 hr (n = 3).

(B-D) Mice received a single dose of PTHrP(1-34) (1 mg/kg body weight; SubQ) and were sacrificed 2 hr later (n = 5-6). Gene expression changes were determined by RT-qPCR. Total UCP1 and ERK1/2 protein levels in inguinal fat tissue were measured by western blotting (D). The values are mean ± SEM. (*) refers to differences compared to the control group. (#) refers to differences between the WT and KO groups. *P < 0.05, ***P < 0.0005, ###P < 0.0005. See also Figure S3.

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