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. Author manuscript; available in PMC: 2017 Feb 9.
Published in final edited form as: Cell Metab. 2015 Dec 6;23(2):315–323. doi: 10.1016/j.cmet.2015.11.003

Figure 7. Adipo-PTHR-KO mice are resistant to LLC tumor-driven cachexia.

Figure 7

(A-D) Mice inoculated with LLC cells were sacrificed 16 days later (n = 6). Carcass weight (calculated by subtracting tumor weight from the total weight) (A) and tumor weight (B) were shown. Fat and muscle tissues were dissected and weighed (C). H&E staining of adipose tissues and gastrocnemius muscle were shown (D).

(E-G) Mice inoculated with LLC cells were sacrificed 14 days later (n = 4-5). mRNA levels in iWAT (E), iBAT (F) were measured by RT-qPCR. Total UCP1 and ERK1/2 protein levels in inguinal fat tissue were determined by western blotting (G).

(H-I) Mice inoculated with LLC cells were sacrificed 16 days later (n = 6). Muscle function was analyzed by grip strength (G). mRNA levels in gastrocnemius muscle (H) were measured by RT-qPCR. The values are mean ± SEM. (*) refers to differences between the LLC and non-tumor-bearing groups. (#) refers to differences between the WT-LLC and KO-LLC groups. *P < 0.05, **P < 0.005, ***P < 0.0005, #P < 0.05, ##P < 0.005, ###P < 0.0005. See also Figure S6.

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