Fig. 1.

Induced lung fibrosis and expression of fibrosis marker proteins. Wild-type C57BL/6J mice received saline vehicle control, silica (2 mg), bleomycin (0.07 mg), or paraquat (0.02 mg) and were killed on day 7 post-exposure. A Total collagen fibers were detected by Masson's Trichrome staining of lung tissue sections, with collagen fibers stained blue (a–d, scale bar 50 μm). Collagen I and III fibers were visualized by Picro-Sirius red staining, with collagen fibers stained red (e–h, scale bar 50 μm). B Collagen I (a–d) and FN1 (e–h) levels were determined by immunohistochemistry on lung sections. Red indicates positive staining, and blue indicates nuclear counterstaining (scale bar 20 μm)