Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
p53 transfection inhibits osteosarcoma cell proliferation. The wild type p53 and green fluorescent protein (GFP) fusion protein expression vector pGFP-p53 was transfected into U-2OS/U-2OSR2 (wild type p53, Wt-p53) and KHOS/KHOSR2 (mutant p53, Mt-p53) cells. Cells were plated in 96-well plates and cultured. Cell proliferation was determined by MTT assay. Results were calculated from five (n=5) repeated experiments with triplicate wells to reflect STD/SEM.
