Fig. 2.

Overexpression of p53 increases Taxol, cisplatin, and doxorubicin sensitivity. The drug cytotoxicity of Taxol, cisplatin, and doxorubicin was assessed in vitro using the MTT assay. 2 × 10³ cells per well were plated in 96-well plates during this process. The second day after transfection, cytotoxic drugs were added. After culturing for 7 days, 50 µl of MTT (5 mg/ml in PBS) was added to each well and incubated for 4 hours. After dissolving the resulting formazan product with acid-isopropanol, the absorbance (A490) was read on a SPECTRAmax Microplate Spectrophotometer at a wavelength of 490 nm. Results were calculated from five (n=5) repeated experiments with triplicate wells to reflect STD/SEM.