Skip to main content
. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Rep. 2016 Jan 28;14(5):1074–1085. doi: 10.1016/j.celrep.2016.01.007

Figure 2.

Figure 2

bmh1Δ cells show premature SAC silencing in response to tension defects. (A) Mad1 phosphorylation kinetics in bmh1Δ cells in the absence of cohesion. MAD1-3HA cells with the indicated genotypes were synchronized in G1 and then released into 37°C YPD medium. The cells were collected over time to examine Mad1 phosphorylation based on the band-shift after western blotting. The budding index and the Mad1 protein levels are shown. (B) The phosphorylation kinetics of Bub1 in bmh1Δ mutant cells in the absence of cohesion. G1-arrested cells with Bub1-13myc were released into YPD at 37°C. Samples were collected periodically and subjected to western blotting with an anti-myc antibody. The budding index and the phosphorylation of Bub1 are shown.