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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Rep. 2016 Jan 28;14(5):1156–1168. doi: 10.1016/j.celrep.2015.12.103

Figure 2. Reducing MacroH2A1.2 Impairs Skeletal Muscle Cell Differentiation.

Figure 2

(A,B) Myogenin protein and mRNA evaluated after siRNA against mH2A1.2 in C2C12 cells. Gapdh and histone H2A were used as loading controls. Data are represented as mean ± SD. (C) Myogenin and (D) myosin heavy chain (MHC) immunofluorescence staining of control (CTRi) and mH2A1.2i C2C12 cells prompted to differentiate for 2 days. DAPI identifies nuclei. (E) mH2A1.2 and Myogenin mRNA expression in C2C12 cells transfected with Flag-empty (CTR) or Flag-mH2A1.2 (f-mH2A1.2) expression vector (0.8 g mH2A1.2 plasmid /1×105 cells). (F) Immunoblot for Flag, Myogenin and Gapdh in C2C12 transfected with Flag-empty (CTR) or Flag-mH2A1.2 vector. Data are represented as mean ± SD.