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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Neurosci Methods. 2015 Dec 29;261:155–160. doi: 10.1016/j.jneumeth.2015.12.008

Figure 4. Frozen sectioning of a gelatin block containing seven half-brain specimens.

Figure 4

The gelatin block was rapidly frozen in a 2-methylbutane/dry ice mixture for approximately 20 seconds and kept in dry ice until mounted on to the microtome stage with O.C.T. compound. The temperature of the block was maintained by the freezing stage temperature controller, and with additional dry ice when needed, during sectioning. A frozen block on the microtome stage is shown (A). A corner of the block was notched for the purpose of identification when working with more than one block (arrow in B).