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. 2015 Aug 17;21(1):666–675. doi: 10.2119/molmed.2015.00187

Figure 2.

Figure 2

Immature leukocyte populations are enriched after burn injury, and hemoglobin is associated with CD45+ positive PBMCs. (A) The expression levels of various bone marrow derived progenitor cell CD markers are significantly elevated in PBMCs of burn patients. Data represent mean ± S.E.M.; n = 6; *p < 0.05. (B) An increased amount of free hemoglobin detected in serum of burn patients. (C) Flow cytometric analysis shows increased CD45+/hemoglobin α+ double positive cells in total PBMC population (from 2% ± 1% in healthy volunteer samples to 16% ± 4% in burn patient samples; n = 3; **p < 0.01.) (D) PBMCs (top subpanel) and U937 cells (middle and bottom subpanel) bind hemoglobin onto the cell surface in a dose-dependent manner. This causes a decrease free hemoglobin content of media in proportion to hemoglobin uptake by U937 cells (bottom subpanel, showing the decrease of total and free hemoglobin from the cell culture media after incubation of the hemoglobin-containing culture medium with U937 cells). Data represent mean with ± S.D.; n = 3; *p < 0.05