Table 1B.

Primer identification, sequences, amplicon length and concentration for the genes investigated

Genes	NM ID	Forward Primer (5’-3’)	Reverse Primer (5’-3’)	Amplicon length bp	Forward primer Final conc. nmol/L	Reverse primer Final conc. nmol/L
References
ACTB	001101	TGGCATCCACGAAAACTAC	GGCAGTGATCTCCTTCTG	142	300	300
PPIA	021130	CGGATTTGATCATTTGGTG	CCAGACAACACACAAGAC	109	300	300
B2M	004048	CCAAAGATTCAGGTTTACTC	CAACTTCAATGTCGGATG	100	600	300
YWHAZ	001135700	AACTGCTTCCATGTCTAA	TTACTACACCTGTGACTG	97	600	300
GAPDH	001289746	GTCGGAGTCAACGGATTT	GCAACAATATCCACTTTACCA	80	600	300
Targets
VEGF-a	001025366	GAGTGGTTGACCTTCC	GGGATATTAAATAAGTACCGTATA	138	300	300
ITGAV	002210	GGGTCAAGATCAGTGAGAAATCTTTAC	ATTCCTGTAACATCATGCTATTGCTAG	139	300	600
ITGB3	00212	CTCCTGTCCCTCATCCATAGC	CAGCCAAGAGGTAGAAGGTAAATAC	103	300	300
CAIX	001216	CTGCCCTCTGACTTCA	CAGAGAGGGTGTGGAG	133	300	300
HIF1-a	181054	AGCAGTCTATTTATATTTTCTACA	GAGCATTAATGTAAATTAAGTAGA	111	300	300
SSTR2	001050	ACCTCGTATAAGCTTCAAG	GGTTCTTCAAATATCTTCTTC	121	600	600
SSTR5	001172560	CTGAGTGGGCACAAATCC	ATTTATCCTCGCATTTATTCAG	121	300	600
uPAR	001005376	GCTGCCTGTGATAAATTATTAC	CTCCCAAAGTGCTAGGAC	116	300	300
mTOR	004958	GATAAGCTCACTGGTCGG	AATATAGCACTGGCAGAGG	114	300	300
Ki67	002417	TCCCGCCTGTTTTCTTTCTGAC	CTCTCCAAGGATGATGATGCTTTAC	121	600	600