Figure 5. Rce1 inhibitors mislocalize H-, N-, and K-Ras in human cells.
(A) HCT-116 cells were transiently transfected with EGFP fusion constructs of H-, N-, or K-Ras, and treated with either DMSO, FTI (2 μM), or Rce1 inhibitors (25 μM) for 20 hours. Images show representative cells. Scale bars = 10 μm. (B-D) Quantification of H-, N-, and K-Ras mislocalization (see supporting information for method). Percent PM association is reported with SEM bars.