Fig. 4.

The concentrations of IL-6 and TNF-α in blood serum and the expression of TNF-α and IL-6 protein immunoreactivity in SG of each group. a Serum concentrations of IL-6 and TNF-α were measured using ELISA. Serum concentrations of IL-6 and TNF-α in the MI group were significantly higher than the Con group, Sham group, MI + BBG group, MI + NONRATT021972 si group, and MI + P2X₇ si group (p < 0.01). There were no differences between Con, Sham, MI + BBG, MI + NONRATT021972 si, and MI + P2X₇ si groups (p > 0.05). There was no difference between the MI group and MI + SC si group (p > 0.05). $\overline{x}\pm s$, n = 6. **p < 0. 01, compared to the Con group; ##p < 0. 01 vs. the MI group. b, c TNF-α expression and IL-6 protein immunoreactivity were detected using immunohistochemistry. Representative photos of TNF-α (b) and IL-6 (c) protein immunoreactivity in SG. Histogram shows the IOD of TNF-α (b) and IL-6 (c) protein immunoreactivity in SG. The expression levels of TNF-α (b) and IL-6 (c) immunoreactivity in the MI group were significantly higher than the Con, Sham, MI + BBG, MI + NONRATT021972 si, and MI + P2X₇ si groups (p < 0.01). No significant differences were observed between the Con, Sham, MI + BBG, MI + NONRATT021972 si, and MI + P2X₇ si groups (p > 0.05). Arrows indicate the immunostained neurons. $\overline{x}\pm s$, n = 10. **p < 0.01, compared to the Con group; ##p < 0. 01 vs. the MI group. Scale bars at 20 μm