Fig. 4.

SNC-Fc reduced intimal thickening in an ex vivo organ culture model.

Representative images of:

a and b: Elastin van Gieson staining to show intimal size.

c and d: Immunohistochemistry for BrdU incorporation (brown nuclei: proliferation).

e and f: ISEL (brown nuclei: apoptosis).

g and h: Immunohistochemistry for QBend10 (brown colour: endothelial cells).

i and j: Dual immunohistochemistry for QBend10 (pink, endothelial cells) and ISEL (brown nuclei, apoptosis).

k and l: Higher power images of the dual QBend10 (pink) and ISEL (brown) to clearly show the double staining in i.

Dotted line delineates the intimal:medial boundary. Arrowheads indicate some of the positive cells.

Scale bars in a-d and g and h represent 20 μm, scale bars in e, f, i, j, k and l represent 40 μm, n = 5.