Fig. 5.
SNC-Fc had no effect HUVEC proliferation and migration.
a: Proliferation (percentage of BrdU positive HUVECs) following 24 h incubation with 20, 100 or 200 pM Fc or SNC-Fc, in the presence of 2% FCS, n = 3. Arrowheads indicate BrdU positive cells (brown), negative cells have blue nuclei (haematoxylin). Scale bars represent 20 μm.
b: Distance migrated in μm after 18 h (scratch wound assay) by HUVECs treated with 20, 100 or 200 pM Fc or SNC-Fc, in the presence of 2% FCS, n = 3. Scale bars represent 100 μm. Arrows indicate the wound edges.