Video 3. Spontaneous global Ca2+ transients and pharmacological treatment during retinal waves.
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DOI: 10.7554/eLife.11583.011
(A) Time-lapse two-photon recording of GCaMP6 signal in a P9 RGC undergoing spontaneous Ca2+ events while participating in retinal waves. (B) A P9 RGC before and during wash-in of 30 mM High K+ mACSF, exhibiting a large sustained increase in GCaMP6 intensity. (C) A P9 RGC before and during wash-in of 5 μM DhβE, exhibiting an abolition of spontaneous wave activity. Scale bars: 5 μm. 0.9 Hz time-lapse, playback speed = 20 frames per second. RGC, retinal ganglion cell.