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. 2015 Dec 9;4:e08497. doi: 10.7554/eLife.08497

Figure 7. Larval muscles regulate AMP proliferation via Insulin-like peptide dIlp6.

(A–I) Flat preparations of the mid-stage matched third instar larvae stained for Twist (green) labeling AMP nuclei and Phalloidin (magenta) labeling larval muscles. One abdominal lateral group of AMPs is shown. (A) Larvae mutant for dIlp6 (Df-Ilp6) shows a lower AMP count. (B, C) No changes in AMP number are observed in dIlp2 or dIlp5 mutant larvae. (D) Muscle-targeted expression of the dominant-negative form of shibire (DN-shi) leads to a decrease in AMP cell number. (E, F) Elav-Gal4-driven expression of DN-shi in neural cells or Repo-Gal4-driven expression in glial cells have no effects on AMP number. (G) Attenuation of dIlp6 in larval muscle leads to a decrease in AMP number while (H) muscle-specific gain-of-function of dIlp6 leads to an increase in AMP number. (I) No change in AMP number is observed after RNAi-based attenuation of dIlp6 in glial cells. (J) Mean number of lateral AMPs counted in different genetic contexts shown in A–I and O. (***) indicates P≤0.001. (K) Posterior lateral AMP revealed by GFP staining (green) of M6-gapGFP second-instar larvae. Arrows indicate long AMP filopodia extending along the segment border muscle (Phalloidin staining, in magenta). (L) A similar view of posterior lateral AMP from second instar DAAM-RNAi larvae. Arrows point to short filopodia. (M) Reduced AMP numbers in third instar larvae induced by M6-targeted attenuation of DAAM. Scale bar in (A–I) and (O): 45 microns; in (M, N): 25 microns.

DOI: http://dx.doi.org/10.7554/eLife.08497.025

Figure 7—source data 1. Table showing mean number of dorsal, lateral and ventral AMPs in the abdominal segments from the genotypes shown in Figure 7A–L and Figure 7—figure supplement 1.
For each genotype, the average number of cells ± standard error mean is shown. Sample size (n) is indicated in brackets.
DOI: http://dx.doi.org/10.7554/eLife.08497.026
elife-08497-fig7-data1.docx (52.1KB, docx)
DOI: 10.7554/eLife.08497.026

Figure 7.

Figure 7—figure supplement 1. Muscle released dIlp6 is required for the activation of dorsal and lateral but not ventral AMPs.

Figure 7—figure supplement 1.

(A, B) Graphical representation of mean number of AMPs from the dorsal and ventral abdominal groups of mid third-instar larvae for the genotypes shown in Figure 7A–L. Notice that muscle released signal is required for activation of both dorsal and ventral AMPs; however, dIlp6, which is required for Insulin pathway activation in lateral and dorsal AMPs, has no effect on ventral AMPs. (***) indicates P≤0.001. P-values were computed using t test and Prism software with different control samples: wt (Canton S) for dIlp deficiencies, Mef-Gal4 for Mef-driven genotypes, Elav-Gal4, for Elav-driven genotypes, and Repo-Gal4 for Repo-driven genotypes.
DOI: http://dx.doi.org/10.7554/eLife.08497.027