Figure 2. Immunolabeling with neuropeptide antibodes on Epon sections.
(A) Representative micrographs with immunogold labeled axons for the neuropeptide antibodies indicated. For the FVa neuropeptide antibody, five adjacent sections are shown (FVa Layer 1–5). (B, C) Neurite-specific labeling in adjacent sections (seven sections apart) labeled with different antibodies. (D–F) High-resolution micrographs of immunogold labeled, silver-enhanced gold particles (arrows), and unenhanced ultra small gold particles (arrowheads) Scale bar: (A-C) 1 μm; (D–F) 200 nm. High-resolution images are available in (Shahidi, et al., 2015).