Figure 9. siGOLD labeling and whole-body neuron reconstructions in HT9-4.
(A, C) Full body IF labeling of RGWa and MIP-positive cells in 72 hpf larvae, anterior views. Arrowheads point to neurons that were traced in EM reconstructions. (B, D) Traced neurons, identified using the siGOLD method in the full body HT9-4 dataset. Dashed arrows indicate immunogold-labeled layers along the neurite, layer number(s) and the number of gold particles per layer are shown. Reconstructed RGWa (B) and MIP (D) positive cells. Scale bars: (A, C) 30 μm.