Table 1.
List of antibodies used
| NP precursor name | Abbreviation | Antigen |
|---|---|---|
| FMRFamide | FMRFa | (C)FMRFa |
| RYamide | RYa | (C)VFRYa |
| Myoinhibitory peptide/Allatostatin B | MIP | (C)AWNKNSMRVWa or (C)VWa |
| RGWamide | RGWa | (C)RGWa or (C)GWa |
| Proenkephalin | PENK | (C)YGDLSFSNSNYa |
| Luqin | LUQ | (C)WRPQGRFa |
| Allatotropin | ATO | (C)GFRTGAYDRFSHGFa |
| Pigment dispersing factor | (C)NPGTLDAVLDMPDLMSLa | |
| Leucokinin | LEUC | (C)KFTPWAa |
| FVamide | FVa | (C)AHRFVa or (C)FVa |
| FVRIamide | FVRIa | (C)FVRIa |
The full name and abbreviation of neuropeptide precursors that contain the neuropeptides used for immunoEM. The FMRFa, RYa, MIP short and long, RGWa, FVa short and long, and FVRIa antibodies have been described previously (Conzelmann and Jékely, 2012; Conzelmann et al., 2011; Conzelmann et al., 2013a; Jékely et al., 2008). All 11 neuropeptides are amidated (a). A Cys (C) was added to the N-terminus of each peptide to allow coupling during immunization and affinity purification. All antibodies were generated in rabbits. For FVa long, a rat antibody was also generated.