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. 2016 Feb 10;11(2):e0148425. doi: 10.1371/journal.pone.0148425

Fig 3. Stress-reactivity measured by novel tank diving in 6-month old adult zebrafish.

Fig 3

A) Time course of average time spent per minute at the bottom of the tank, B) overall average time spent per minute at the bottom of the tank each minute, C) mean distance travelled per minute during novel tank diving. D) Effect of diazepam on zebrafish stress-reactivity assessed by novel tank diving. A,B. Zebrafish that had been developmentally exposed to ethanol showed reduced bottom dwelling (F 2, 682 = 3.47, P<0.05). There were no significant differences in distance travelled (C). D. Diazepam also significantly reduced time spent by adults at the bottom of the tanks compared to controls (F 1, 408 = 5.45, P<0.001). Post hoc t-test, *** P<0.001.